Description
Accession
A0A2K5WJ66
Source
DNA sequence encoding Cynomolgus IL-10 R alpha including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus IL-10 R alpha is a protein consisting of amino acid residues [His22-Asn233] ,The protein has a predicted MW of 25.34 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus IL-10 R alpha was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus IL-10 R alpha Protein Tris-Bis PAGE
- Cynomolgus IL-10 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus IL-10 R alpha Protein SEC-HPLC
- The purity of Cynomolgus IL-10 R alpha is greater than 95% as determined by SEC-HPLC.
Cynomolgus IL-10 R alpha Protein ELISA Data
- Immobilized Human IL-10, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus IL-10 R alpha, His Tag with the EC50 of 8.9ng/ml determined by ELISA.
Cynomolgus IL-10 R alpha Protein SPR Data
- Cynomolgus IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-10, His Tag with an affinity constant of 0.93 nM as determined in SPR assay (Biacore T200).
