Cynomolgus GARP&Latent TGF beta 1 Complex Protein Recombinant
Category: Receptors$212.00 – $708.00
Description
Accession
A0A2K5X2X9
Source
DNA sequence encoding Cynomolgus GARP&Latent TGF beta 1 Complex including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus GARP&Latent TGF beta 1 Complex is a protein consisting of amino acid residues [Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)] ,The protein has a predicted MW of 70.5 kDa (GARP)&41.3 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF Beta 1) based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus GARP&Latent TGF beta 1 Complex was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus GARP&Latent TGF beta 1 Complex Protein Tris-Bis PAGE
- Cynomolgus GARP&Latent TGF beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus GARP&Latent TGF beta 1 Complex Protein SEC-HPLC
- The purity of Cynomolgus GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC.
Cynomolgus GARP&Latent TGF beta 1 Complex Protein ELISA Data
- Immobilized Cynomolgus GARP&Latent TGF beta 1 Complex, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GARP&Latent TGF Beta 1 Antibody, hFc Tag with the EC50 of 11.0ng/ml determined by ELISA.
