Description
Accession
G7PL88
Source
DNA sequence encoding Cynomolgus CTLA-4/CD152 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus CTLA-4/CD152 is a protein consisting of amino acid residues [Ala37-Ser160] ,The protein has a predicted MW of 14.4 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus CTLA-4/CD152 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus CTLA-4/CD152 Protein Tris-Bis PAGE
- Cynomolgus CTLA-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus CTLA-4/CD152 Protein SEC-HPLC
- The purity of Cynomolgus CTLA-4 is greater than 95% as determined by SEC-HPLC.
Cynomolgus CTLA-4/CD152 Protein ELISA Data
- Immobilized Cynomolgus CTLA-4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA.
Cynomolgus CTLA-4/CD152 Protein SPR Data
- Cynomolgus B7-1, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 9.90 nM as determined in SPR assay (Biacore T200).
