Cynomolgus CLEC12A/MICL/CLL-1 Protein Recombinant
Category: Receptors$220.00 – $708.00
Description
Accession
A0A2K5WXQ6
Source
DNA sequence encoding Cynomolgus CLEC12A/MICL/CLL-1 including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus CLEC12A/MICL/CLL-1 is a protein consisting of amino acid residues [His65-Ala265] ,The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus CLEC12A/MICL/CLL-1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus CLEC12A/MICL/CLL-1 Protein Tris-Bis PAGE
- Cynomolgus CLEC12A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus CLEC12A/MICL/CLL-1 Protein SEC-HPLC
- The purity of Cynomolgus CLEC12A is greater than 95% as determined by SEC-HPLC.
Cynomolgus CLEC12A/MICL/CLL-1 Protein ELISA Data
- Anti-CLEC12A Antibody captured on CM5 Chip via Protein A can bind Cynomolgus CLEC12A, His Tag with an affinity constant of 1.28 μM as determined in SPR assay (Biacore T200).
