Description
Accession
A0A2K5US85
Source
DNA sequence encoding Cynomolgus CD83 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus CD83 is a protein consisting of amino acid residues [Thr20-Glu147] ,The protein has a predicted MW of 15.43 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus CD83 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus CD83 Protein Tris-Bis PAGE
- Cynomolgus CD83 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus CD83 Protein SEC-HPLC
- The purity of Cynomolgus CD83 is greater than 95% as determined by SEC-HPLC.
Cynomolgus CD83 Protein ELISA Data
- Immobilized Cynomolgus CD83, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti- CD83 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
