Description
Accession
A0A2K5WCS2
Source
DNA sequence encoding Cynomolgus CD46 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus CD46 is a protein consisting of amino acid residues [Cys35-Asp329] ,The protein has a predicted MW of 34.05 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus CD46 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus CD46 Protein Tris-Bis PAGE
- Cynomolgus CD46 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus CD46 Protein SEC-HPLC
- The purity of Cynomolgus CD46 is greater than 95% as determined by SEC-HPLC.
Cynomolgus CD46 Protein ELISA Data
- Immobilized Cynomolgus CD46, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD46 Antibody, hFc Tag with the EC50 of 0.67μg/ml determined by ELISA.
