Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein Recombinant
Category: Receptors$253.00 – $843.00
Description
Accession
Q95LI5
Source
DNA sequence encoding Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma is a protein consisting of amino acid residues [Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)] ,The protein has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein Tris-Bis PAGE
- Cynomolgus CD3E&CD3G on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein SEC-HPLC
- The purity of Cynomolgus CD3E&CD3G was greater than 95% as determined by SEC-HPLC.
Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein ELISA Data
- Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with EC50 of 51.2ng/ml determined by ELISA.
