Cynomolgus CA9/Carbonic Anhydrase IX Protein Recombinant
Category: Receptors$220.00 – $708.00
Description
Accession
A0A2K5VQG9
Source
DNA sequence encoding Cynomolgus CA9/Carbonic Anhydrase IX including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus CA9/Carbonic Anhydrase IX is a protein consisting of amino acid residues [Gln38-Asp402] ,The protein has a predicted MW of 41.03 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus CA9/Carbonic Anhydrase IX was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus CA9/Carbonic Anhydrase IX Protein Tris-Bis PAGE
- Cynomolgus CA9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus CA9/Carbonic Anhydrase IX Protein SEC-HPLC
- The purity of Cynomolgus CA9 is greater than 95% as determined by SEC-HPLC.
Cynomolgus CA9/Carbonic Anhydrase IX Protein ELISA Data
- Immobilized Cynomolgus CA9, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA.
