Description
Accession
XP_005578557
Source
DNA sequence encoding Cynomolgus B7-H6/NCR3LG1 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus B7-H6/NCR3LG1 is a protein consisting of amino acid residues [Asp25-Asp259] ,The protein has a predicted MW of 27.6 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus B7-H6/NCR3LG1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus B7-H6/NCR3LG1 Protein Tris-Bis PAGE
- Cynomolgus B7-H6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus B7-H6/NCR3LG1 Protein SEC-HPLC
- The purity of Cynomolgus B7-H6 is greater than 95% as determined by SEC-HPLC.
Cynomolgus B7-H6/NCR3LG1 Protein SPR Data
- Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H6, His Tag with an affinity constant of 0.662 μM as determined in SPR assay (Biacore T200).
