Description
Accession
A0A1D5Q330
Source
DNA sequence encoding Cynomolgus AXL including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus AXL is a protein consisting of amino acid residues [Glu33-Pro449] ,The protein has a predicted MW of 46.3 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus AXL was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus AXL Protein Tris-Bis PAGE
- Cynomolgus AXL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus AXL Protein SEC-HPLC
- The purity of Cynomolgus AXL is greater than 95% as determined by SEC-HPLC.
Cynomolgus AXL Protein ELISA Data
- Immobilized Cynomolgus AXL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA.
Cynomolgus AXL Protein SPR Data
- Cynomolgus Axl, His Tag immobilized on CM5 Chip can bind Cynomolgus GAS6, His Tag with an affinity constant of 1.70 nM as determined in SPR assay (Biacore T200).
