//Cynomolgus ALCAM/CD166 Protein Recombinant

Cynomolgus ALCAM/CD166 Protein Recombinant

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$220.00$708.00

SKU: KTBWIERH

Description

Accession
G7NZQ8
Source
DNA sequence encoding Cynomolgus ALCAM/CD166 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus ALCAM/CD166 is a protein consisting of amino acid residues [Trp28-Lys527] ,The protein has a predicted MW of 57.10 kDa. Due to glycosylation, the protein migrates to 70-85 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus ALCAM/CD166 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Methods

Cynomolgus ALCAM/CD166 Protein Tris-Bis PAGE

  • Cynomolgus ALCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Cynomolgus ALCAM/CD166 Protein SEC-HPLC

  • The purity of Cynomolgus ALCAM is greater than 95% as determined by SEC-HPLC.

Cynomolgus ALCAM/CD166 Protein ELISA Data

  • Immobilized Cynomolgus ALCAM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.62μg/ml determined by ELISA.
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