Description
Accession
A0A8C0TNP6
Source
DNA sequence encoding Canine GDF15 including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Canine GDF15 is a protein consisting of amino acid residues [Ala197-Val307] ,The protein has a predicted MW of 13.12 kDa. The protein migrates to 15-20 kDa under reduced (R) condition and 23-26 kDa under Non reducing (N) condition based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Canine GDF15 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Canine GDF15 Protein Tris-Bis PAGE
- Canine GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.
Canine GDF15 Protein ELISA Data
- Immobilized Canine GDF15, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 48.3ng/ml determined by ELISA.
Canine GDF15 Protein ELISA Data
- Immobilized Canine GDF15, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
