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Bovine Noggin Recombinant
Categories: Bovine growth factorsFood grade growth factors$400.00 – $1,900.00
Description
Accession
G3N3L0
Source
Optimized DNA sequence encoding Bovine Noggin mature chain sp|RKG3N3L0|28-232AA was expressed in Insect Cells.
Molecular weight
Recombinant Bovine Noggin is a protein consisting of 205 amino acid residue subunits, due to glycosylation migrates as an approximately 35kDa protein under reducing conditions in SDS-PAGE.
Purity
>96%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined inhibit BMP-4-induced alkaline phosphatase production by mouse ATDC5 cells. The ED50-for this effect is 2-100 ng/mL in the presence of 50 ng/mL of recombinant Human BMP‑4.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Bovine Noggin was lyophilized from a 0.2 μm filtered solution in PBS, pH.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
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