Biotinylated Human IL-5 Protein Recombinant
Categories: Biotinylated cytokinesBiotinylated proteins$542.00 – $1,805.00
Description
Accession
P05113
Source
DNA sequence encoding Human IL-5 including a N-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human IL-5 is a protein consisting of amino acid residues [Ile20-Ser134] ,The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-5 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Molecular function
Molecular function
Methods
Biotinylated Human IL-5 Protein Tris-Bis PAGE
- Biotinylated Human IL-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human IL-5 Protein SEC-HPLC
- The purity of Biotinylated Human IL-5 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-5 Protein ELISA Data
- Immobilized Biotinylated Human IL-5, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 39ng/ml determined by ELISA.
Biotinylated Human IL-5 Protein SPR Data
- Human IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Human IL-5, His-Avi Tag with an affinity constant of 7.57 nM as determined in SPR assay (Biacore T200).
