///Human Interferon-gamma Recombinant (CHO Cell)

Human Interferon-gamma Recombinant (CHO Cell)

Category:

$160.00$1,250.00

SKU: RCP01579 Tags: , , , ,

Description

Accession
P01579
Source
Optimized DNA sequence encoding Human Interferon gamma mature chain was expressed in CHO Cells
Molecular weight
Native Interferon gamma is generated by the proteolytic removal of the signal peptide , the molecule has a calculated molecular mass of approximately 17 kDa. Recombinant Interferon gamma is a monomer protein consisting of 143 amino acid residue subunits, due to glycosylation migrates as an approximately 25 kDa protein under reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by a cytotoxicity assay of HT29 cells and was found to be ≤ 0.5ng/ml, corresponding to a specific activity of ≥2x107 units/mg.
Protein Sequence
MKYTSYILAF QLCIVLGSLG CYCQDPYVKE AENLKKYFNA GHSDVADNGT LFLGILKNWK EESDRKIMQS QIVSFYFKLF KNFKDDQSIQ KSVETIKEDM NVKFFNSNKK KRDDFEKLTN YSVTDLNVQR KAIHELIQVM AELSPAAKTG KRKRSQMLFR GRRASQ
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant Interferon gammawas lyophilized from a.2μm filtered concentrated (1mg/ml) solution in PBS, pH.2.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Q66793
Interactor
Biological Process
Biological Process
Molecular function
Stay in the loop
For new product information and regular updates on our product range please fill your details
Loading