Mouse R-Spondin1 Recombinant
Category: Recombinant Mouse Cytokines$160.00 – $2,750.00
Description
Accession
Q9Z132
Source
Optimized DNA sequence encoding peptidase domain of Mouse R-Spondin1(SER21-GLN265 ) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight
Recombinant Mouse R-Spondin-1 is a protein consisting of 250 amino acid residue subunits,due to glycosylation migrates as an approximately 45kDa protein on reduced SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity was tested by the ability recombinant mouse RSPO1 to induce activation of beta catenin in Luciferase assay in the presence of recombinant mouse Wnt3a, the calulated ED50 is 50-200 ng/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse R-spondin1 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage
RecombinantR-spondin1can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Methods
Isolation of intestinal crypts
- Tissue was removed from PBS solution and washed multiple times with ice-cold PBS washes until the solution remained clear.
- The specimen was then placed in a Petri dish containing PBS on ice with the mucosal surface facing upward.
- Using a razor blade, excess mucoid material was scrapped from the epithelial surface.
- The specimen was then divided into approximately 0.5 cm2 pieces.
- These pieces were placed into a 2.5 mmol/L EDTA solution in PBS for 30 minutes of incubation with gentle shaking at 4°C.
- After this incubation period, the fragments were allowed to settle and the supernatant was discarded.
- 10 ml of cold PBS was added to the sample, and subsequently vortexed for 10 seconds with 1-second bursts.
- The fragments were allowed to settle, and the supernatant was removed and saved on ice.
- Again 10 ml of PBS were added and the process was repeated eight times.
- Samples were spun down at 100 g for 2 minutes.
- The supernatant…
