Description
Accession
P06879
Source
Optimized DNA sequence encoding Mouse Prolactin mature chain fused to a C terminal polyHis tag was expressed in Insect Cells.
Molecular weight
Native mouse Prolactin is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 23kDa. Recombinant Prolcatin is a monomeric protein consisting of 200 amino acid residue subunits, and migrates as an approximately 23kDa protein under non-reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity was determined by the dose-dependent proliferation of Nb2 cells.
Protein Sequence
MNSQGSAQKA GTLLLLLISN LLFCQNVQPL PICSAGDCQT SLRELFDRVV ILSHYIHTLY TDMFIEFDKQ YVQDREFMVK VINDCPTSSL ATPEDKEQAL KVPPEVLLNL ILSLVQSSSD PLFQLITGVG GIQEAPEYIL SRAKEIEEQN KQLLEGVEKI ISQAYPEAKG NGIYFVWSQL PSLQGVDEES KILSLRNTIR CLRRDSHKVD NFLKVLRCQI AHQNNC
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse Prolactin is presented as concentratedmg/ml,.2 μm filteredmM Tris solution,0.5M NaCl pH.4 containing% trehalose% glycerol.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration of.1 mg/mL.
Storage
Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
