Description
Accession
P10889
Source
Optimized DNA sequence encoding mouse GRO-beta CXCL2 mature chain was expressed in Escherichia Coli.
Molecular weight
Native mouse GRO-beta is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8kDa. Recombinant GRO-beta is a monomer protein consisting of 73 amino acid residue subunits, and migrates as an approximately 8kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract transfected CXCR2 cells using a concentration range of-100 ng/ml.
Protein Sequence
MAPPTCRLLS AALVLLLLLA TNHQATGAVV ASELRCQCLK TLPRVDFKNI QSLSVTPPGP HCAQTEVIAT LKGGQKVCLD PEAPLVQKII QKILNKGKAN
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant GRO-beta/CXCL2 was lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution inmM Tris, pH7.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Q2F862
Biological Process
Biological Process
Molecular function
Methods
Rab27a promotes migration of neutrophils in vivo and in vitro.
- Leukocytes recruited to the bronchoalveolar space of WT and Rab27a KO mice treated intranasally with MIP-2; cells were stained with antibody against Ly6G and analysed by flow cytometry.
Rab27a promotes migration of neutrophils in vivo and in vitro.
- Leukocytes recruited to the bronchoalveolar space of WT and Rab27a KO mice treated intranasally with MIP-2; cells were stained with antibody against Ly6G and analysed by flow cytometry.
