Human TECK (CCL25 ) Recombinant
Categories: CCL chemokinesRecombinant Human Cytokines$70.00 – $2,700.00
Description
Accession
O15444
Source
Optimized DNA sequence encoding Human CCL25 mature chain was expressed in Escherichia Coli.
Molecular weight
Native human CCL25 has a calculated molecular mass of approximately 14 kDa. Recombinant CCL25 is a monomer protein consisting of 128 amino acid residue subunits, and migrates as an approximately 14 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
Activity was determined by the ability to induce chemotaxis of purified human monocytes at concentrations ranging between-10 ng/ml.
Protein Sequence
MNLWLLACLV AGFLGAWAPA VHTQGVFEDC CLAYHYPIGW AVLRRAWTYR IQEVSGSCNL PAAIFYLPKR HRKVCGNPKS REVQRAMKLL DARNKVFAKL HHNTQTFQAG PHAVKKLSSG NSKLSSSKFS NPISSSKRNV SLLISANSGL
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant CCL25 was lyophilized from PBS pH.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
P51686
Biological Process
Biological Process
Molecular function
Methods
Serum CCL25 levels in LuCa patients Serum CCL25 levels in healthy donors (n=9), SCC (n=17) and AC (n=14) patients were analyzed by ELISA.
- As used, the ELISA could detect >5 pg/mL of CCL25.
