Human LYN Recombinant
Categories: CytokinesRecombinant Human Cytokines$160.00 – $1,950.00
Description
Accession
P07948
Source
DNA sequence encoding extracellular domain of Human LYN to a N-terminal GST tag was expressed in Insect cells.
Molecular weight
Recombinant Human LYN is a monomer consisting of 736 amino acid residue , and migrates as an approximately 75 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE
Biological Activity
The activity was determined using a Glu/Tyr peptide substrate in a fluorogenic assay , and was determined to be 100nM/min/mg.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human LYN was lyophilized from a 0.2 μm filtered 20mM Tris,50mM NaCl, pH 8.0, 0.5mM PMSF.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P06241
Interactor
P22681
Interactor
P43405
Interactor
Interactor
Q9HCN6
Biological Process
Biological Process
Biological Process
Molecular function
