Mouse Interleukin-25 Recombinant
Category: Recombinant Mouse Cytokines$70.00 – $4,700.00
Description
Accession
Q9CPT4
Source
Optimized DNA sequence encoding Mouse Interleukin-25 (MYGDF) mature chain was expressed in Escherichia Coli.
Molecular weight
Native Mouse IL-25 has a calculated molecular mass of approximately 16 kDa. Recombinant IL-25 is a monomeric protein consisting of 143 amino acid residue subunits, andmigrates as an approximately 16 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Activitywas determined by the ability to bind mouse IL17RB with a linear range of-40 ng/ml in functional ELISA assay
Protein Sequence
MAAPSGGFWT AVVLAAAALK LAAAVSEPTT VPFDVRPGGV VHSFSQDVGP GNKFTCTFTY ASQGGTNEQW QMSLGTSEDS QHFTCTIWRP QGKSYLYFTQ FKAELRGAEI EYAMAYSKAA FERESDVPLK SEEFEVTKTA VSHRPGAFKA ELSKLVIVAK AARSEL
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse Interleukin-25 was lyophilized from.2μm filtered concentrated (1.0mg/ml) solution in PBS, pH7.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process