//Human CD106 Recombinant (VCAM1)

Human CD106 Recombinant (VCAM1)

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$160.00$1,950.00

SKU: RKP19320 Tags: , , , , ,

Description

Accession
P19320
Source
Optimized DNA sequence encoding Human extracellular domain of human CD106including a C-terminal 6His tag was expressed in HEK293 cells.
Molecular weight
Recombinant CD106 (VCAM1) is a monomer protein consisting of689 amino acid residue subunits,due to glycosylation migrates as an approximately110 kDa protein on SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity of recombinant VCAM1 was tested by immobilized recombinant CD106 (10ug/ml) ability to support adhesion of U937 human histiocytic lymphoma cells (5x104 cells/well ) , 70% will adhere after 60min at 37C.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant CD106is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
Storage
Recombinant CD106, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process

Methods

Flow cytometry and Western analysis

  • surface expression was evaluated by staining with phycoerythrin (PE)-conjugated anti-VCAM-1 (eBioscience, 12-1069-41), goat polyclonal anti-IL13α2 AF146 followed by detection with PE-conjugated donkey anti-goat , PE-conjugated mouse monoclonal anti-IL13α2 B-13 ( lots 1107403, 1107404 and 1107405), or unconjugated B-13 antibodies ( and ) followed by detection with PE-conjugated goat anti-mouse ( Immunoesearch).
  • In some cases B-13 reagent was first pre-incubated for thirty minutes with soluble recombinant human IL13α2-Fc chimera or VCAM-1/C106-Fc chimera reagents .
  • Percentages of positively staining cells were calculated using the subtraction method with FCS Express version 3 software .
  • Relative fluorescence intensity was calculated by dividing the MFI experimental by the MFI of the control.
  • IL-13 biotin binding assays were done by biotinylating IL-13 at a 2.5 biotin to recombinant IL-13 ratio using the EZ-Link Sulfo-NHS-Biotin kit as per the manufacturer's instructions.
  • The biotinylated IL-13 was then incubated with various glioma lines, followed by staining with
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