/, CCL chemokines/Human Eotaxin-1 (CCL11 ) Recombinant

Human Eotaxin-1 (CCL11 ) Recombinant

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$70.00$2,700.00

SKU: RKP51671 Tags: , , ,

Description

Accession
P51671
Source
Optimized DNA sequence encoding Human Eotaxin-1 mature chain was expressed in Escherichia Coli.
Molecular weight
Native human Eotaxin-1 has a calculated molecular mass of approximately 8 kDa. Recombinant Eotaxin-1 is a monomer protein consisting of 74 amino acid residue subunits, andmigrates as an approximately 8 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
Activitywas determined by the ability to induce chemotaxis of purified eosinophils at concentrations ranging between-100 ng/ml.

Protein Sequence
MKVSAALLWL LLIAAAFSPQ GLAGPASVPT TCCFNLANRK IPLQRLESYR RITSGKCPQK AVIFKTKLAK DICADPKKKW VQDSMKYLDQ KSPTPKP
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant Eotaxin-1 wasis lyophilized from as.2μm filtered concentrated (1.0mg/ml) PBS solution, pH.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P49682
Interactor
P51677
Interactor
P51681
Biological Process
Biological Process
Molecular function

Methods

SPR sensorgrams for binding of hCCR3 with its ligands.

  • The sensorgrams show the binding of hCCR3 with CCL11 and CCL24 in the presence of FC-14 and the binding of hCCR3 with CCL11 and CCL24 in the presence of DDM.
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