Human beta Defensin-4 Recombinant
Categories: PDGF familyPDGF familyRecombinant Human Cytokines$70.00 – $2,700.00
Description
Accession
Q8WTQ1
Source
Optimized DNA sequence encoding Humanbeta Defensin-4 mature chain was expressed in Escherichia Coli.
Molecular weight
Native humanBD-4 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant beta Defensin-4 is a monomer protein consisting of51 amino acid residue subunits, and migrates as an approximately kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by its ability to chemoattract immature human dendritic cells using a concentration range of-50 ng/ml.
Protein Sequence
MQRLVLLLAI SLLLYQDLPV RSEFELDRIC GYGTARCRKK CRSQEYRIGR CPNTYACCLR KWDESLLNRT KP
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
RecombinantHuman betaDefensin-4 was lyophilized from.2 μm filtered100mM NaCl,mM PB, pH.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function