Human Myostatin Recombinant
Categories: TGF-beta familyTGF-beta family$70.00 – $4,700.00
Description
Accession
O14793
Source
Optimized DNA sequence encoding Human Myostatin mature chain was expressed in Escherichia Coli.
Molecular weight
Native human Myostatin, generated by the proteolytic removal of the signal peptide and propeptide, molecule has a calculated molecular mass of approximately 13 kDa. Recombinant human Myostatin is a disulfide-linked homodimeric protein consisting of two 110 amino acid residue subunits, and migrates as an approximately 25 kDa protein under non-reducing conditions and as 13 kDa protein under reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependentproliferation inhibition of human MPC-11 cells was found to be in the range of.0-40.0 ng/ml.
Protein Sequence
MQKLQLCVYI YLFMLIVAGP VDLNENSEQK ENVEKEGLCN ACTWRQNTKS SRIEAIKIQI LSKLRLETAP NISKDVIRQL LPKAPPLREL IDQYDVQRDD SSDGSLEDDD YHATTETIIT MPTESDFLMQ VDGKPKCCFF KFSSKIQYNK VVKAQLWIYL RPVETPTTVF VQILRLIKPM KDGTRYTGIR SLKLDMNPGT GIWQSIDVKT VLQNWLKQPE SNLGIEIKAL DENGHDLAVT FPGPGEDGLN PFLEVKVTDT PKRSRRDFGL DCDEHSTESR CCRYPLTVDF EAFGWDWIIA PKRYKANYCS GECEFVFLQK YPHTHLVHQA NPRGSAGPCC TPTKMSPINM LYFNGKEQII YGKIPAMVVD RCGCS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Myostatin was lyophilized from a 0.2 μm filtered Tris solutionpH7.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Molecular function
Molecular function
