Rat Interleukin-5 Recombinant
Categories: HematopoietinsIL-3 familyRecombinant Rat Cytokines$70.00 – $4,700.00
Description
Accession
Q08125
Source
Optimized DNA sequence encoding Rat Interleukin-5 mature chain was expressed in Escherichia Coli.
Molecular weight
Native Rat Interleukin-5, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa. Recombinant IL-5 is a disulfide-linked homodimeric protein consisting of two 113 amino acid residue subunits,and migrates as an approximately 26 kDa protein under non-reducing conditions and as a 13 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent proliferation of TF-1 cells was ≤.2 ng/ml, corresponding to a specific activity of ≥ x units/mg.
Protein Sequence
MRMLLCLNVL TLSCVWAIAM EIPMSTVVKE TLIQLSTHRA LLTSNETMRL PVPTHKNHQL CIGEIFQGLD ILKNQTVRGG TVEILFQNLS LIKKYIDGQK EKCGEERRKT RHFLDYLQEF LGVMSTEWAM EV
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant rat Interleukin-5 was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only. It may not be used for therapeutics or diagnostic purposes.
Molecular function
Molecular function
Methods
γIMCs are the source of IFN-γ in severe invasive GAS infections.
- (f) Sorted IMCs were cultured with control medium (Med), G-CSF (50 ng ml−1), M-CSF (10 ng ml−1), GM-CSF (10 ng ml−1), or IL-5 (10 ng ml−1) for 2–4 days, and their absolute numbers were counted on the indicated days.
