Human Interleukin-15 Recombinant, Biotinylated
Categories: Biotinylated proteinsCytokinesRecombinant Human Cytokines$160.00 – $3,500.00
Description
Accession
P40933
Source
DNA sequence encoding Human Interleukin-15 mature chain fused to an AVI IgG1 C-terminal tag was expressed in HEK292 cells.
Molecular weight
Native human Interleukin-15 is generated by the proteolytic removal of the signal peptide and propeptide with a theoretical molecular mass of approximately 13kDa. Recombinant Interleukin-15 Fc chimera is a homodimer protein and migrates an approximately as a 41KDa protein under reducing conditions in SDS-PAGE.
Purity
>96%, as determined by SDS-PAGE and HPLC
Biological Activity
Activity was determined by the binding of human IL-15 receptor alpha chain in a functional ELISA assay in a linear range of 1-100 ng/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant IL-15 was lyophilized from a 0.2 μm filtered PBS solution pH7.5 .
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Molecular function
