///Human Growth Hormone Recombinant 192AA

Human Growth Hormone Recombinant 192AA

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$70.00$300.00

SKU: RKP01241 Tags: , , , , ,

Description

Accession
P01241
Source
Optimized DNA sequence encoding Human Growth Hormone mature chain was expressed in Escherichia Coli.
Molecular weight
Native human placental Growth Hormone is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 20 kDa. Recombinant Growth Hormone is a monomeric protein consisting of 192 amino acid residue subunits.GH migrates as an approximately 20 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkeyMBr-5 cells was found to be in the range of.0-40.0 ng/ml.

Protein Sequence
MATGSRTSLL LAFGLLCLPW LQEGSAFPTI PLSRLFDNAM LRAHRLHQLA FDTYQEFNPQ TSLCFSESIP TPSNREETQQ KSNLELLRIS LLLIQSWLEP VQFLRSVFAN SLVYGASDSN VYDLLKDLEE GIQTLMGRLE DGSPRTGQIF KQTYSKFDTN SHNDDALLKN YGLLYCFRKD MDKVETFLRI VQCRSVEGSC GF
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Growth Hormonewas lyophilized from a.2 μm filtered.005 mM NaHCO3 solution pH.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
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