bovine Basic Fibroblast Growth Factor Recombinant
Categories: Bovine growth factorsFGF family$120.00 – $500.00
Description
Accession
P03969
Source
Optimized DNA sequence encoding Bovine Basic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli.
Molecular weight
Native bovine bFGF is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximately kDa. Recombinant bovine basic FGF is a monomer protein, consisting of amino acids and migrates as an approximately kDa protein under reducing conditions.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the proliferation ofNR6R-3T3 fibroblasts, was found to be less than.1 ng/ml,corresponding tox106 IU/mg.
Protein Sequence
MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGPKTG PGQKAILFLP MSAKS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant bovine bFGFwas lyophilized from a.2 μm filtered PBS solution pH7.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function
