Optimized DNA sequence encoding rat Interleukin-6 mature chain was expressed in Escherichia Coli.
Native rat Interleukin-6 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 22 kDa.
Recombinant rat Interleukin-6 is a disulfide-linked monomer protein consisting of 188 amino acid residue subunits, and migrates as an approximately 22 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant rat Interleukin-6 was lyophilized from a 0.2 μm filtered PBS solution.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
IL6 is produced by many
different cell types. The
main sources in vivo are
fibroblasts, and endothelial
cells. Macrophages, T-cells
granulocytes, smooth muscle
mast cells, glial cells, and
keratinocytes also produce
IL6 after stimulation.
IL6 is a member of a family
of cytokines, which also
includes LIF, CNTF,
Oncostatin M, IL11, and CT-
1. All known members of the
IL6 cytokine family induce
hepatic expression of acute
The IL6 receptor is
expressed on T-cells,
peripheral monocytes and
some macrophage- and B-cell
derived tumor cell types. It
is not expressed in resting
B-cells but in resting T-
cells. The IL6 receptor is a
protein of 80 kDa and a
length of 449 amino acids.
It has been designated
IL-6 protects against hyperoxia-induced mitochondrial damage via bcl-2–induced bak interactions with mitofusions
Am. J. Respir. Cell Mol. Biol.,
385 - 396.
long-term safety and efficacy of tocilizumab, an anti-IL-6 receptor monoclonal antibody, in monotherapy, in patients with rheumatoid arthritis (the stream study): evidence of safety and efficacy in a 5-year extension study
Ann Rheum Dis,
1580 - 1584.
the second bovine β-galactoside-2,6-sialyltransferase (st6gal ii): genomic organization and stimulation of its in vitro expression by IL-6 in bovine mammary epithelial cells
1082 - 1093.
IL-6 -174g/c promoter polymorphism in acute appendicitis
600 - 603.
cell surface-bound il-1 is an upstream regulator of the senescence-associated IL-6/il-8 cytokine network