Optimized DNA sequence encoding rat Interleukin-6 mature chain was expressed in Escherichia Coli.
Molecular weight:
Native rat Interleukin-6 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 22 kDa.
Recombinant rat Interleukin-6 is a disulfide-linked monomer protein consisting of 188 amino acid residue subunits, and migrates as an approximately 22 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity:
>95%, as determined by SDS-PAGE and HPLC
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation:
Recombinant rat Interleukin-6 was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
IL-6
IL6 is produced by many
different cell types. The
main sources in vivo are
stimulated monocytes,
fibroblasts, and endothelial
cells. Macrophages, T-cells
and B-lymphocytes,
granulocytes, smooth muscle
cells, eosinophils,
chondrocytes, osteoblasts,
mast cells, glial cells, and
keratinocytes also produce
IL6 after stimulation.
IL6 is a member of a family
of cytokines, which also
includes LIF, CNTF,
Oncostatin M, IL11, and CT-
1. All known members of the
IL6 cytokine family induce
hepatic expression of acute
phase proteins.
The IL6 receptor is
expressed on T-cells,
mitogen-activated B-cells,
peripheral monocytes and
some macrophage- and B-cell
derived tumor cell types. It
is not expressed in resting
B-cells but in resting T-
cells. The IL6 receptor is a
strongly glycosylated
protein of 80 kDa and a
length of 449 amino acids.
It has been designated
CD126.
Related Publications:
IL-6 protects against hyperoxia-induced mitochondrial damage via bcl-2–induced bak interactions with mitofusions
Am. J. Respir. Cell Mol. Biol.,
Oct 2009;
41:
385 - 396.
long-term safety and efficacy of tocilizumab, an anti-IL-6 receptor monoclonal antibody, in monotherapy, in patients with rheumatoid arthritis (the stream study): evidence of safety and efficacy in a 5-year extension study
Ann Rheum Dis,
Oct 2009;
68:
1580 - 1584.
the second bovine β-galactoside-2,6-sialyltransferase (st6gal ii): genomic organization and stimulation of its in vitro expression by IL-6 in bovine mammary epithelial cells
Glycobiology,
Oct 2009;
19:
1082 - 1093.
IL-6 -174g/c promoter polymorphism in acute appendicitis
Lab Med,
Oct 2009;
40:
600 - 603.
cell surface-bound il-1 is an upstream regulator of the senescence-associated IL-6/il-8 cytokine network
PNAS,
Sep 2009;
10.1073/pnas.0905299106.
