| Source: |
| Optimized DNA sequence encoding Rat Interleukin-1 beta mature chain was expressed in Escherichia Coli. |
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| Molecular weight: |
|
Mature Rat IL-1 beta, is generated by the proteolytic removal of the signal peptide
and propeptide.The molecule has a calculated molecular mass of approximately 17 kDa.
Recombinant IL-1 beta is a disulfide-linked monomer protein consisting of two 153 amino acid residue subunits. Recombinant IL-1 beta migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
|
|
| Purity: |
| >98%, as determined by SDS-PAGE and HPLC |
|
| Biological Activity: |
| The ED(50) was determined by the dose-dependent stimulation of thymidine uptake by murine D10S cells is ≤ 2.0 pg/ml, corresponding to a specific activity of ≥5 x 108 units/mg. |
|
| Protein Sequence: |
10 20 30 40 50 60
MATVPELNCE IAAFDSEEND LFFEADRPQK IKDCFQALDL GCPDESIQLQ ISQQHLDKSF
70 80 90 100 110 120
RKAVSLIVAV EKLWQLPMSC PWSFQDEDPS TFFSFIFEEE PVLCDSWDDD DLLVCDVPIR
130 140 150 160 170 180
QLHCRLRDEQ QKCLVLSDPC ELKALHLNGQ NISQQVVFSM SFVQGETSND KIPVALGLKG
190 200 210 220 230 240
LNLYLSCVMK DGTPTLQLES VDPKQYPKKK MEKRFVFNKI EVKTKVEFES AQFPNWYIST
250 260 270
SQAEHRPVFL GNSNGRDIVD FTMEPVSS |
(*)Complete precursor sequence shown, expressed chain highlighted
|
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
|
| Presentation: |
| Rat IL-1 beta was lyophilized from a 0.2 μm filtered solution in PBS, pH 7. |
|
| Reconstitution: |
| A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
|
| Storage: |
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles. |
|
| Usage: |
| This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
|
