Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 µg/ml.
Immunohistochemical analysis of FFPE mouse Hippocampus sections expressing beta NGF.
Cat# RPA01138 was used as primary antibody, dilution 1:500, HE staining.
Source:
Polyclonal beta NGFantibody was produced from sera of rabbits immunized with synthetic protein containing beta nerve growth factor antigenic determinants.
Species reactivity:
Human,Mouse,Rat
Purification:
Serum IgG fraction was purified by Protein G affinity chromatography.
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
Storage:
The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Usage:
This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
beta NGF
NGF is mainly responsible
for the survival and the
differentiation and the
functional activities of
sensory and sympathetic
neurons in the peripheral
nervous system. It also
plays an important role in
the development and
functional activities of
cholinergic neurons in the
central nervous system.
Since NGF is synthesized
also in non-neuronal tissues
it may have a much wider
spectrum of biological
activities than thought
previously. NGF stimulates
chemotactic migration of
human polymorphonuclear
leukocytes in vitro. NGF
stimulates the growth and
differentiation of B-cells
and the growth of T-cells
and of some tumor cell
types. NGF inhibits
immunoglobulin production by
various human plasma cell.
The cytokines IL1, IL6, and
bFGF are potent inducers of
NGF. NGF induces the
synthesis of IL1 in
pheochromocytoma cells which
in turn acts as a growth
factor for glial cells and
induces the synthesis of NGF
following nerve injuries. In
thymic stromal cells NGF
induces the synthesis of
IL6. NGF induces the
synthesis of the fos
oncogene and the myc
oncogene and also influences
the expression of EGF. One
receptor that is responsible
for mediating most of the
activities of NGF is
expressed preferentially in
neuronal tissues. This
glycoprotein of 140 kDa is
the product of the trk gene.
It possesses an intrinsic
tyrosine-specific protein
kinase in its intracellular
domain.
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