Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 µg/ml.
Immunohistochemical analysis of PPFE rat hippocampus tissue expressing PP2A.
Cat# RPA67775 was used as primary antibody, dilution 1:200.
Source:
Polyclonal Protein Phosphatase 2A-PP2A antibody was produced from sera of mice immunized with synthetic protein containingPP2A antigenic determinants.
Species reactivity:
Human,Mouse,Rat
Purification:
Serum IgG fraction was purified by Protein G affinity chromatography.
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
Storage:
The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Usage:
This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
PP2A
Related Publications:
β1-integrin-collagen interaction suppresses foxo3a by the coordination of akt and PP2A J. Biol. Chem.,
May 2010;
285:
14195 - 14209.
the b563 regulatory subunit of PP2A regulates s phase-specific nuclear accumulation of PP2A and the g1 to s transition
J. Biol. Chem.,
May 2010;
10.1074/jbc.M109.094953.
protein phosphatase 2a (PP2A) holoenzymes regulate death-associated protein kinase (dapk) in ceramide-induced anoikis
J. Biol. Chem.,
Apr 2010;
285:
13827 - 13838.
neuroprotectin d1 induces dephosphorylation of bcl-xl in a PP2A-dependent manner during oxidative stress and promotes retinal pigment epithelial cell survival
J. Biol. Chem.,
Apr 2010;
10.1074/jbc.M109.095232.
pr65, the heat-repeat scaffold of phosphatase PP2A, is an elastic connector that links force and catalysis
PNAS,
Feb 2010;
107:
2467 - 2472.
