| Applications: |
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Elisa: Use at a concentration range of 1-2ug/ml. |
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Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml. |
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IHC: A suitable range of concentrations of this antibody for IHC is 2-10 µg/ml. |
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| Source: |
| Polyclonal PDGF-B antibody was produced from sera of rabbits immunized with synthetic protein containing PDGF-B antigenic determinants. |
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| Species reactivity: |
| Human,Mouse,Rat |
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| Purification: |
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Serum IgG fraction was purified by Protein G affinity chromatography.
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| Presentation: |
| Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml. |
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| Reconstitution: |
| A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers |
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| Storage: |
The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles. |
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| Usage: |
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This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
PDGF-B |
PDGF is synthesized mainly
by megakaryocytes. It is
stored in the alpha granules
of platelets from which it
is released after cell
activation of platelets.
Platelets synthesize a
mixture of the three
possible isoforms
(BB,AB,AA)while fibroblasts
stimulated with EGF
synthesize AA homodimers.
PDGF receptors are expressed
in fibroblasts, osteoblasts,
chondroblasts, smooth muscle
cells, glial cells, and
endothelial cells.Two
related receptors, are
PDGFRalpha (CD140a) or
PDGFRbeta ( CD140b).In
contrast to many other
cytokines PDGF is not
released into the
circulation. PDGF binds to
several plasma proteins and
also to proteins of the
extracellular matrix which
facilitates local
concentration of the factor.
The factor functions as a
local autocrine and
paracrine growth factor. In
the adult organism PDGF is
involved in wound healing
processes. The aberrant
expression of PDGF is
observed with vascular
proliferative diseases such
as atherosclerosis.
PDGF regulates the synthesis
of its own receptor and also
influences the expression of
membrane receptors for IL1,
EGF, 5-Hydroxytryptamine,
LDL, transferrin, and
muscarinergic receptor.
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