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Rabbit anti IL-8 HRP Polyclonal

IL-8

accession:  P10145
   Size A     100ug   $ 320
   Size B       $
   Size C       $
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA10145H
Applications:
Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC-P/F: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
 
IL-8 IHC

Immunohistochemical analysis of IL-8 expression mouse ovarian vascular endothelial cells.

Cat# RPA10145 was used as primary antibody, dilution 1:200.

 
Source:
Polyclonal Interleukin-8 antibody was produced from sera of rabbits  immunized with synthetic protein containing Interleukin-8  antigenic determinants. 
 
Species reactivity:
Mouse,Rat
 
Purification:

Serum IgG fraction was purified by Protein A affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

IL-8

IL8 is produced by stimulated monocytes but not by tissue macrophages and T- lymphocytes. In many cell types the synthesis of IL8 is strongly stimulated by IL1 and TNF- alpha. In human skin fibroblasts the expression of IL-8 is enhanced by Leukoregulin.The expression of IL-8 from resting and stimulated human blood monocytes is up-regulated by IL-7. In chondrocytes the synthesis of IL8 is stimulated by IL1-beta TNF- alpha and bacterial lipopolysaccharides. In human astrocytes the synthesis and secretion of IL8 is induced by IL-1 and TNF-alpha. The IL8 receptor is a dimeric glycoprotein consisting of a 59 kDa and a 67 kDa subunit. It has been given the name CD128. It is expressed in many different cell types including those not responding to IL8. The biological activities of IL8 resemble those of a related protein, NAP-2. IL8 differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. In neutrophils IL8 causes a transient increase in cytosolic calcium levels and the release of enzymes from granules. IL-8 also enhances the metabolism of reactive oxygen species and increases Chemotaxis and the enhanced expression of adhesion molecules. A pre-activation by IL3 is required to render basophils and neutrophils susceptible to further activation by IL8.

Related Publications:
cell surface-bound il-1 is an upstream regulator of the senescence-associated il-6/IL-8 cytokine network
PNAS, Sep 2009; 10.1073/pnas.0905299106.
the angiogenic effect of probiotic bacillus polyfermenticus on human intestinal microvascular endothelial cells is mediated by IL-8
Am J Physiol Gastrointest Liver Physiol, Sep 2009; 10.1152/ajpgi.00204.2009.
IL-8 signaling does not mediate intra-amniotic lps-induced inflammation and maturation in preterm fetal lamb lung
Am J Physiol Lung Cell Mol Physiol, Sep 2009; 297: L512 - L519.
the functional significance behind expressing two IL-8 receptor types on pmn
J. Leukoc. Biol., Sep 2009; 86: 529 - 543.
IL-8 regulates mucin gene expression at the posttranscriptional level in lung epithelial cells
J. Immunol., Aug 2009; 183: 2159 - 2166.




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Antibodies:
Purified Polyclonal
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