Cart contains    items
SEARCH

  |
  |  
  |  
  |  
  |  
  |  
 
Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Rabbit anti IL-3 Polyclonal

IL-3

accession:  P08700
   Size A     100ug   $ 190
   Size B      200ug  $290
   Size C      500ug  $ 490
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA08700
Applications:
il-2 elisa Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
 
Source:
Polyclonal Interleukin-3 antibody was produced from sera of rabbits  immunized with synthetic protein containing Interleukin-3 antigenic determinants. 
 
Species reactivity:
Mouse,Rat
 
Purification:

Serum IgG fraction was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

IL-3

IL3 is produced mainly by T- cells following cell activation by antigens and mitogens, but also by keratinocytes, NK-cells, mast cells, endothelial cells, and monocytes. The analysis of bacterial- derived recombinant IL3 shows that glycosylation is not required for the activity of IL3. IL3 sequences are evolutionarily less well conserved. Human and murine IL3 show approximately 29 % homology at the protein level while murine and rat IL3 show approximately 54 % homology. IL3-alpha and IL3- beta are two isoforms of rat IL3. IL3 receptors are expressed on macrophages, mast cells, eosinophils, megakaryocytes, basophils, bone marrow progenitor cells, and various myeloid leukemia cells. IL3/receptor complexes have a Kdis of 10-9 - 10-10 M. Binding of IL3 to its receptor causes specific phosphorylation of a 150 kDa membrane glycoprotein.

Related Publications:
a conserved insulator that recruits ctcf and cohesin exists between the closely related but divergently regulated Interleukin-3 and granulocyte-macrophage colony-stimulating factor genes
Mol. Cell. Biol., Apr 2009; 29: 1682 - 1693.
a new isoform of Interleukin-3 receptor with novel differentiation activity and high affinity binding mode
J. Biol. Chem., Feb 2009; 284: 5763 - 5773.
identification of 24p3 as a direct target of foxo3a regulated by Interleukin-3 through the phosphoinositide 3-kinase/akt pathway
J. Biol. Chem., Jan 2009; 284: 2187 - 2193.
Interleukin-3 (il-3)-induced c-fos activation is modulated by gab2-calcineurin interaction
J. Biol. Chem., Aug 2008; 283: 23505 - 23509.
Interleukin-3 promotes expansion of hemopoietic-derived cd45+ angiogenic cells and their arterial commitment via stat5 activation
Blood, Jul 2008; 112: 350 - 361.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human Interleukin-3 Recombinant
product_icon_8.jpg Human Interleukin-31 Recombinant
product_icon_8.jpg Mouse Interleukin-3 Recombinant
product_icon_7.jpg Rabbit anti Human IL32 alpha Polyclonal
product_icon_8.jpg Human Interleukin-33 Recombinant
product_icon_8.jpg Human Interleukin-3 Recombinant (CHO cell)
product_icon_7.jpg Rabbit anti IL-3 Polyclonal
product_icon_8.jpg Mouse Interleukin-33 Recombinant
product_icon_8.jpg Human IL-31R beta Recombinant (OSMR)
product_icon_8.jpg Human IL-35 Recombinant
product_icon_8.jpg Human IL-3 receptor Recombinant (CD123)
product_icon_8.jpg Human IL-3RB Recombinant (CD131)
product_icon_8.jpg Mouse IL-31R beta Recombinant (OSMRb)
 
 
 
Item name Size Quantity Price(USD) Remove
checkout
Cart Total $