Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
WB analysis of certoli cell lysate expressing FASL.
Cat# RPA48023 was used as primary antibody.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 µg/ml.
Source:
Polyclonal anti-FAS Ligand (FASL) antibody was produced from sera of rabbits immunized with synthetic protein containing FAS Ligand antigenic determinants.
Species reactivity:
Human,Mouse,Rat
Purification:
Serum IgG fraction was purified by Protein G affinity chromatography.
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
Storage:
The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Usage:
This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
FASL
Related Publications:
single nucleotide polymorphisms in fas and FASL and sensitivity to gefitinib in patients with advanced non-small cell lung cancer.
ASCO Meeting Abstracts,
May 2010;
28:
e13529.
FASL gene knock-down therapy enhances the antiglioma immune response
Neuro Oncology,
May 2010;
12:
482 - 489.
extreme lymphoproliferative disease and fatal autoimmune thrombocytopenia in FASL and trail double-deficient mice
Blood,
Apr 2010;
115:
3258 - 3268.
microglia are neurotoxic or neuroprotective during the development of glaucoma depending upon the expression of membrane and soluble FASL Invest. Ophthalmol. Vis. Sci.,
Apr 2010;
51:
3190.
peptide vaccination breaks tolerance to her-2/neu by generating vaccine-specific FASL+ cd4+ t cells: first evidence for intratumor apoptotic regulatory t cells
Cancer Res.,
Apr 2010;
70:
2686 - 2696.
