Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
WB analysis of certoli cell lysate expressing FASL.
Cat# RPA48023 was used as primary antibody.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 µg/ml.
Polyclonal anti-FAS Ligand (FASL) antibody was produced from sera of rabbits immunized with synthetic protein containing FAS Ligand antigenic determinants.
Serum IgG fraction was purified by Protein G affinity chromatography.
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
single nucleotide polymorphisms in fas and FASL and sensitivity to gefitinib in patients with advanced non-small cell lung cancer.
ASCO Meeting Abstracts,
FASL gene knock-down therapy enhances the antiglioma immune response
482 - 489.
extreme lymphoproliferative disease and fatal autoimmune thrombocytopenia in FASL and trail double-deficient mice
3258 - 3268.
microglia are neurotoxic or neuroprotective during the development of glaucoma depending upon the expression of membrane and soluble FASL Invest. Ophthalmol. Vis. Sci.,
peptide vaccination breaks tolerance to her-2/neu by generating vaccine-specific FASL+ cd4+ t cells: first evidence for intratumor apoptotic regulatory t cells
2686 - 2696.