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Rabbit anti CD20 Polyclonal

CD20

accession:  P11836
   Size A     100ug   $ 190
   Size B      200ug  $290
   Size C      500ug  $ 490
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA11836
Applications:
Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
 
CD20 Immunohistochemistry/IHC

Immunohistochemical analysis of FFPE Human colon cancer sections expressing CD20. 
Cat# RPA11836 was used as primary antibody, dilution 1:500, 
DAB chromogenic substrate.

 

 

 

CD20 Immunohistochemistry/IHC

Immunohistochemical analysis of FFPE mouse groin lymphoma sections expressing CD20. 
Cat# RPA11836 was used as primary antibody, dilution 1:500, DAB chromogenic substrate.

 

 

 

 
Source:
Polyclonal CD20 antibody was produced from sera of rabbits  immunized with synthetic protein containing CD20 antigenic determinants. 
 
Species reactivity:
Human,Mouse,Rat
 
Purification:

Serum IgG fraction was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CD20

CD20 is a signature B-cell differentiation antigen strongly expressed on the surface of mature normal as well as malignant B cells. It is also expressed, but at lower levels and with larger variance, on more immature B cells and their malignant counterparts found in B-cell precursor (BCP) acute lymphoblastic leukemia. The precise function of CD20 remains unknown, it appears to act as part of an ion channel expressed in the plasma membrane,recently shown that CD20 may operate as a store operated calcium (Ca2+) channel facilitating entry of extracellular Ca2+ following BCR-induced emptying of intracellular stores. Interestingly, despite this presumably important role, CD20- deficient mice are without obvious phenotype, which suggests that either this function is not critical for B cell physiology or that molecular redundancy exists.

Related Publications:
antigenic modulation limits the efficacy of anti-CD20 antibodies: implications for antibody selection
Blood, Jun 2010; 115: 5191 - 5201.
increasing the efficacy of CD20 antibody therapy through the engineering of a new type ii anti-CD20 antibody with enhanced direct and immune effector cell–mediated b-cell cytotoxicity
Blood, Jun 2010; 115: 4393 - 4402.
antifungal therapy with itraconazole impairs the anti-lymphoma effects of rituximab by inhibiting recruitment of CD20 to cell surface lipid rafts
Cancer Res., Jun 2010; 70: 4292 - 4296.
expanded cd23+/cd21hi b cells in inflamed lymph nodes are associated with the onset of inflammatory-erosive arthritis in tnf-transgenic mice and are targets of anti-CD20 therapy
J. Immunol., Jun 2010; 184: 6142 - 6150.
CD20-immunotargeting of malignant melanoma.
ASCO Meeting Abstracts, May 2010; 28: 8565.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
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by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
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