Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 µg/ml.
Source:
Polyclonal BMP-7 antibody was produced from sera of rabbits immunized with synthetic protein containing BMP-7 antigenic determinants.
Species reactivity:
Human,Mouse,Rat
Purification:
Serum IgG fraction was purified by Protein G affinity chromatography.
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
Storage:
The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Usage:
This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
BMP-7
BMP-7(osteogenic protein-
1)is produced in the kidney
and in the nervous system.
It is produced also by human
fetal trophoblasts in vivo
and may be involved in the
regulation of the synthesis
of chorionic gonadotropin
and progesterone by
trophoblasts. BMP7 induces
new bone growth, and forms
heterodimers with BMP2A. The
factor is a member of the
TGF family of proteins.
Related Publications:
differential regulation of e-cadherin and -smooth muscle actin by bmp 7 in human renal proximal tubule epithelial cells and its implication in renal fibrosis.
Am J Physiol Renal Physiol,
Sep 2009;
10.1152/ajprenal.90539.2008.
heparin cross-linked collagen coated tcp/ha granules as a carrier for BMP-7 J Bone Joint Surg Br Proceedings,
Sep 2009;
91-B:
445.
BMP-7 fails to attenuate tgf-β1-induced epithelial-to-mesenchymal transition in human proximal tubule epithelial cells
Nephrol. Dial. Transplant.,
May 2009;
24:
1406 - 1416.
the treatment of recalcitrant long bone non-unions with bone morphogenetic protein-7 (BMP-7)
J Bone Joint Surg Br Proceedings,
May 2009;
91-B:
268.
BMP-7 induces wound healing without neovascularization and opacification in a corneal alkali injured mouse model
Invest. Ophthalmol. Vis. Sci.,
Apr 2009;
50:
4549.
