Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
WB analysis mouse hippocampal tissue lysate expressing BCL-2.
Cat# RPA10415 was used as primary antibody.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 µg/ml.
Immunohistochemical analysis of PPFE rabbit hippocampal tissue expressing BCL-2.
Cat# RPA10415 was used as primary antibody, dilution 1:200.
Immunohistochemical analysis of PPFE human endometrial tissue expressing BCL-2.
Cat# RPA10415 was used as primary antibody, dilution 1:500.
Polyclonal BCL-2antibody was produced from sera of mice immunized with synthetic protein containing BCL-2 antigenic determinants.
Serum IgG fraction was purified by Protein G affinity chromatography.
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
akt activation emulates chk1 inhibition and BCL2 overexpression and abrogates g2 cell cycle checkpoint by inhibiting brca1 foci
J. Biol. Chem.,
ifn restores breast cancer sensitivity to fulvestrant by regulating stat1, ifn regulatory factor 1, nf-b, BCL2 family members, and signaling to caspase-dependent apoptosis
Mol. Cancer Ther.,
1274 - 1285.
analysis of pro-apoptotic BCL2 family member initiators bim, bid, and bbc3 in retinal ganglion cell death
Invest. Ophthalmol. Vis. Sci.,
BCL2 and human papilloma virus 16 as predictors of outcome following concurrent chemoradiation for advanced oropharyngeal cancer
Clin. Cancer Res.,
2138 - 2146.
BCL2 is not required for the development and maintenance of leukaemia stem cells in mice