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Rabbit Anti IL-4 Polyclonal

IL-4

accession:  P05112
   Size A     100ug   $ 190
   Size B      200ug  $290
   Size C      500ug  $ 490
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA051121

 

Applications:
il-2 elisa Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
   
Source:
Polyclonal Interleukin-4 antibody was produced from sera of rabbits  immunized with synthetic protein containing C- terminal Interleukin-4 antigenic determinants. 
 
Species reactivity:
Mouse,Rat
 
Purification:

Serum IgG fraction was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

IL-4

Interleukin-4 is produced mainly by a subpopulation of activated T-cells (Th2) which are the biologically most active helper cells for B-cells and which also secrete IL-5 and IL6. The biological activities of IL-4 are mediated by a specific receptor The extracellular domain of the IL4 receptor is related to the receptors for EPO, IL6, and the beta chain of the IL2 receptor. It has been given the name CD124. Two types of IL4 receptor (IL4R) exist: the type 1 receptor is a heterodimer consisting of CD132 and IL4R-alpha. The type 2 receptor is a heterodimer consisting of IL4R-alpha and IL13R-alpha- 1. IL4 enhances expression of MHC class 2 antigens on B- cells. It can promote their capacity to respond to other B-cell stimuli and to present antigens for T- cells.Pretreatment of macrophages with IL4 prevents the production of IL1, TNF-alpha and prostaglandins in response to activation of the cells by bacterial endotoxins or IFN-gamma. IL4 synergises with EPO and G-CSF in the generation of colonies containing granulocytes or erythroid progenitor cells in a colony formation assay.

Related Publications:
pathogenicity of a disease-associated human IL-4 receptor allele in experimental asthma
J. Exp. Med., Sep 2009; 206: 2191 - 2204.
complex regulation of tartrate-resistant acid phosphatase (trap) expression by interleukin 4(IL-4): IL-4 indirectly suppresses receptor activator of nf -kappab ligand (rankl)- mediated trap expression but modestly induces its expression directly
J. Biol. Chem., Sep 2009; 10.1074/jbc.M109.001016.
gm-csf and IL-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through m-csf receptor shedding by up-regulation of tnf- converting enzyme (tace)
Blood, Sep 2009; 10.1182/blood-2009-04-215020.
ship represses the generation of il-3-induced m2 macrophages by inhibiting IL-4 production from basophils
J. Immunol., Sep 2009; 183: 3652 - 3660.
mycobacterium tuberculosis culture filtrate proteins plus cpg-odn confer protection to bcg primed mice by inhibiting IL-4 secretion
Infect. Immun., Sep 2009; 10.1128/IAI.00580-09.




by class:
Cytokines
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Growth factors
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by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
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