Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of IL-1b (10 µg/ml), a concentration of 4-8 µg/ml of this antibody is required.
ELISA:This antibody can be used at 1 μg/mL with the appropriate secondary reagents to detect human VEGF.
Source:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human IL-1 beta.
Species reactivity:
Human
Purification:
The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
Presentation:
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in PBS pH 7.4.
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage:
The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Usage:
This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
IL-1 beta
Monocytes are the main
source of secreted IL1. They
express predominantly IL1-
beta while human
keratinocytes express large
amounts of IL1-alpha. Murine
macrophages display a
transition from IL1-beta to
IL1-alpha production during
maturation of monocytes into
inflammatory
macrophages.There are two
functionally almost
equivalent forms of IL1,
IL1-alpha and IL1-beta that
are encoded by two different
genes. IL1-beta is the
predominant form in humans
while it is IL1-alpha in
mice.
Both forms of IL1 bind to
the same receptor and
therefore also show similar
if not identical biological
activities. The IL1-beta but
not the IL1-alpha precursor
must be processed before it
can bind to the receptor.
Both forms of IL1 bind to
the same receptor and
therefore also show similar
if not identical biological
activities. The receptor
isolated from T-cells is
expressed predominantly on
T-cells and cells of
mesenchymal origin. It binds
both types of IL1 with equal
affinity. This type is
called also Type 1 receptor.
It has been designated
CD121a.
The Type 2 receptor has been
designated CD121b. It is
isolated from B-cells,
granulocytes, and
macrophages. It is expressed
predominantly on B-cells and
cells of the myelomonocytic
lineage and is encoded by a
separate gene.
Related Publications:
cell surface-bound il-1 is an upstream regulator of the senescence-associated il-6/il-8 cytokine network
PNAS,
Sep 2009;
10.1073/pnas.0905299106.
expression of bfgf and IL-1 alpha in tears of galactosemic rats: implications for wound healing and neovascularization
Invest. Ophthalmol. Vis. Sci.,
Apr 2009;
50:
545.
cutting edge: critical role for mesothelial cells in necrosis-induced inflammation through the recognition of il-1 released from dying cells
J. Immunol.,
Dec 2008;
181:
8194 - 8198.
influence of interleukin 1 (il-1), il-4, and il-6 polymorphisms on genetic susceptibility to chronic osteomyelitis
Clin. Vaccine Immunol.,
Dec 2008;
15:
1888 - 1890.
role of acute and prolonged IL-1 alpha & beta induction on primary culture of primary porcine culture
Invest. Ophthalmol. Vis. Sci.,
Apr 2008;
49:
1657.
