Western blot:This monoclonal antibody has been tested in WB analysis of human CD4 . A suitable range of concentrations of this antibody for WB detection is 0.5-2 µg/ml.
Source:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human CD4.
Species reactivity:
Human
Purification:
The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
Presentation:
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in citrate-phosphate buffer pH7.0, with 0.02%Proclin 300 as a preservative.
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage:
The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Usage:
This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
CD4
CD4 is a 55 kDa
transmembrane glycoprotein
belonging to the
immunoglobulin superfamily.
In humans CD4 is expressed
of peripheral T-cells,
thymocytes, on macrophages
and granulocytes.
CD4 acts as a cellular
adhesion molecule binding
MHC class 2 molecules. It
stabilizes the interaction
of MHC class 2 restricted T-
cells and antigen-presenting
cells expressing an antigen
in combination with a MHC
class 2 molecule.
Interactions of CD4 with MHC
class 2 molecules are
crucial during thymic
development and subsequently
for the function of single-
positive CD4(+), CD8(-) T-
lymphocytes.
CD4 functions as an effector
molecule for intracellular
signal transduction. In T-
lymphocytes CD4 has been
found to be associated with
tyrosine kinase lck which
phosphorylates a number of
other cell surface proteins
and is involved probably in
transducing the
intracellular signal when
these proteins are bound to
their receptors.
Related Publications:
CD4+cd25+ regulatory t cells resist a novel form of cd28- and fas-dependent p53-induced t cell apoptosis
J. Immunol.,
Jan 2010;
184:
94 - 104.
CD4+ cd25+ foxp3+ t regulatory cells with limited tcr diversity in control of autoimmunity
J. Immunol.,
Jan 2010;
184:
56 - 66.
early CD4+ t cell help prevents partial cd8+ t cell exhaustion and promotes maintenance of herpes simplex virus 1 latency
J. Immunol.,
Jan 2010;
184:
277 - 286.
evaluation of CD4-CD4i antibody architectures yields potent, broadly cross-reactive anti-human immunodeficiency virus reagents
J. Virol.,
Jan 2010;
84:
261 - 269.
leptin: a critical regulator of CD4+ t-cell polarization in vitro and in vivo
Endocrinology,
Jan 2010;
151:
56 - 62.
