Optimized DNA sequence encoding mature chain of Mouse Angiopoietin-1 including an C-terminal His tag was expressed in HEK293 cells.
Recombinant Angpt1 is a protein consisting of 484 amino acid residue subunits, due to glycosylation migrates as an approximately 70kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Mouse Angiopoietin-1 is lyophilized from 0.2 μm filtered 20mM SOdium Phosphate, pH7.5 ,200mM NaCl, 5% Trehalose.
A quick spin of the vial followed by gently mixing in distilled water to prepare a stock solution concentration not less than 0.1 mg/mL.
Avoid vigorous shaking or vortex
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, the protein can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months, addition of a carrier protein to your working aliquots (0.1% BSA or HSA) is recommended for long term storage
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Angiopoietin-2 (Ang2)2 is a
70-kDa secreted ligand whose
increased expression has
been implicated in a range
of diseases, including
cancer, sepsis, and acute
syndrome. The primary
receptor for Ang2 is the
kinase Tie2 (3) that is
expressed mainly on vascular
endothelial cells and
myeloid cells (1, 4). Ang2
plays an important role in
vascular remodeling during
development, but in adult
tissues, Ang2 concentrations
are usually low. An increase
in Ang2 levels in disease
allows the molecule to
compete for binding to a
common interface on Tie2
with the related agonist
Angiopoietin-2–induced arterial stiffness in ckd
J. Am. Soc. Nephrol.,
endothelial cell-derived Angiopoietin-2 controls liver regeneration as a spatiotemporal rheostat
416 - 419.
Angiopoietin-2 secretion by endothelial cell exosomes: regulation by the phosphatidylinositol 3-kinase (pi3k)/akt/endothelial nitric oxide synthase (enos) and syndecan-4/syntenin pathways
J. Biol. Chem.,
510 - 519.
thrombospondin-1 induction in the diabetic myocardium stabilizes the cardiac matrix in addition to promoting vascular rarefaction through Angiopoietin-2 upregulation
1331 - 1344.
circulating Angiopoietin-2, its soluble receptor tie-2, and mortality in the general population
Eur J Heart Fail,
1327 - 1334.