/, Cytokines/Human Adiponectin Recombinant

Human Adiponectin Recombinant

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$160.00$3,500.00

SKU: RKQ15848 Tags: , , , , , , ,

Description

Accession
Q15848
Source
Optimized DNA sequence encoding extracellular domain of Human Adiponectin (19-244) including a C-terminal His tag was expressed in CHO cells.
Molecular weight
Recombinant Adiponectin is a protein consisting of 226 amino acid residue subunits, due to glycosylation migrates as an approximately 35kDa protein on SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity was tested by Determined by a cytotoxic assay using M1 cells. The ED50 for this effect is 3.0-6.0 μg/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Adiponectin is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage
Recombinant Adiponectin can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function

Methods

Globular adiponectin induces apoptosis in HepG2 cells.

  • Cells were treated with gAcrp (1 μg/ml) for the indicated time points.
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