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Human Noggin Recombinant (CHO Cell)

Noggin

accession:  Q13253
   Size A     10ug   $ 160
   Size B      50ug  $480
   Size C      1mg  $ 3500
Domain  : 
Gene  :  NOG
Catalog no. :  RCQ13253

Source:
Optimized DNA sequence encoding Human Noggin mature chain was expressed in CHO Cells

 
Molecular weight:

Native Human Noggin, generated by the proteolytic removal of the signal peptide
and propeptide,the molecule has a calculated molecular mass of approximately 23 kDa.

Recombinant Human Noggin is a protein consisting of 206 amino acid residue subunits,  migrates as an approximately 30 kDa protein under reducing conditions in SDS-PAGE.


 
Purity:
>96%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by induced alkaline phosphatase secretion in ATDC cellsand was determined to be ≤ 20 ng/ml, corresponding to a specific activity of ≥ 5x 105 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MERCPSLGVT LYALVVVLGL RAAPAGGQHY LHIRPAPSDN LPLVDLIEHP DPIFDPKEKD 

        70         80         90        100        110        120 
LNETLLRSLL GGHYDPGFMA TSPPEDRPGG GGGPAGGAED LAELDQLLRQ RPSGAMPSEI 

       130        140        150        160        170        180 
KGLEFSEGLA QGKKQRLSKK LRRKLQMWLW SQTFCPVLYA WNDLGSRFWP RYVKVGSCFS 

       190        200        210        220        230 
KRSCSVPEGM VCKPSKSVHL TVLRWRCQRR GGQRCGWIPI QYPIISECKC SC 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Human Noggin was lyophilized from a 0.2 μm filtered solution in PBS, pH 7.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Noggin



Related Publications:
identification of a key residue mediating bone morphogenetic protein (bmp)-6 resistance to Noggin inhibition allows for engineered bmps with superior agonist activity
J. Biol. Chem., Apr 2010; 285: 12169 - 12180.
Noggin protects against ischemic brain injury in rodents
Stroke, Feb 2010; 41: 357 - 362.
bone morphogenetic protein antagonist Noggin promotes skin tumorigenesis via stimulation of the wnt and shh signaling pathways
Am. J. Pathol., Sep 2009; 175: 1303 - 1314.
in vitro and in vivo imaging of antivasculogenesis induced by Noggin protein expression in human venous endothelial cells
FASEB J, Dec 2009; 23: 4126 - 4134.
role of Noggin as an upstream signal in the lack of neuronal derivatives found in the avian caudal-most neural crest
Development, May 2009; 136: 1717 - 1726.




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Related Products:
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product_icon_8.jpg Human Noggin Recombinant (CHO Cell)
 
 
 
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