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Human Interleukin-1 alpha Recombinant (CHO Cell)

IL-1

accession:  P01583
   Size A     5ug   $ 160
   Size B      25ug  $480
   Size C      1mg  $ 4700
Domain  :  Interleukin-1
Gene  :  IL1A
Catalog no. :  RCP01583
Source:
Optimized DNA sequence encoding Human Interleukin-1 alpha mature chain was expressed in CHO Cells.

 
Molecular weight:

Mature human IL-1 alpha, is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 18 kDa.

Recombinant Interleukin-1a is a disulfide-linked monomer protein consisting of 160 amino acid residue subunits, due to glycosylation migrates as an approximately  22kDa protein under reducing conditions in SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of murine D10S cells is ≤ 0.002 ng/ml, corresponding to a specific activity of ≥ 2 x 109 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MAKVPDMFED LKNCYSENEE DSSSIDHLSL NQKSFYHVSY GPLHEGCMDQ SVSLSISETS 

        70         80         90        100        110        120 
KTSKLTFKES MVVVATNGKV LKKRRLSLSQ SITDDDLEAI ANDSEEEIIK PRSAPFSFLS 

       130        140        150        160        170        180 
NVKYNFMRII KYEFILNDAL NQSIIRANDQ YLTAAALHNL DEAVKFDMGA YKSSKDDAKI 

       190        200        210        220        230        240 
TVILRISKTQ LYVTAQDEDQ PVLLKEMPEI PKTITGSETN LLFFWETHGT KNYFTSVAHP 

       250        260        270 
NLFIATKQDY WVCLAGGPPS ITDFQILENQ A 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Interleukin-1 alpha was lyophilized from a 0.2 μm filtered  PBS solution pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-1 alpha

Monocytes are the main source of secreted IL1. They express predominantly IL1- beta while human keratinocytes express large amounts of IL1-alpha. Murine macrophages display a transition from IL1-beta to IL1-alpha production during maturation of monocytes into inflammatory macrophages.There are two functionally almost equivalent forms of IL1, IL1-alpha and IL1-beta that are encoded by two different genes. IL1-beta is the predominant form in humans while it is IL1-alpha in mice. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL1-beta but not the IL1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.

Related Publications:
cell surface-bound il-1 is an upstream regulator of the senescence-associated il-6/il-8 cytokine network
PNAS, Sep 2009; 10.1073/pnas.0905299106.
expression of bfgf and IL-1 alpha in tears of galactosemic rats: implications for wound healing and neovascularization
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 545.
cutting edge: critical role for mesothelial cells in necrosis-induced inflammation through the recognition of il-1 released from dying cells
J. Immunol., Dec 2008; 181: 8194 - 8198.
influence of interleukin 1 (il-1), il-4, and il-6 polymorphisms on genetic susceptibility to chronic osteomyelitis
Clin. Vaccine Immunol., Dec 2008; 15: 1888 - 1890.
role of acute and prolonged IL-1 alpha & beta induction on primary culture of primary porcine culture
Invest. Ophthalmol. Vis. Sci., Apr 2008; 49: 1657.




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Antibodies:
Purified Polyclonal
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