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Human Interleukin-15 Recombinant (CHO Cell)

IL-15

accession:  P40933
   Size A     5ug   $ 160
   Size B      25ug  $480
   Size C      1mg  $ 3500
Domain  :  Interleukin-15
Gene  :  IL15
Catalog no. :  RCP40933
Source:
Optimized DNA sequence encoding Human Interleukin-15 mature chain was expressed in CHO Cell.

 
Molecular weight:
Native human Interleukin-15 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa.

Recombinant Interleukin-15 is a  protein consisting of  114 amino acid residue subunits, due to glycosylation migrates as an approximately 15kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>96%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent  proliferation of mouse CTLL-2 cells and was found less than 0.3 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MRISKPHLRS ISIQCYLCLL LNSHFLTEAG IHVFILGCFS AGLPKTEANW VNVISDLKKI 

        70         80         90        100        110        120 
EDLIQSMHID ATLYTESDVH PSCKVTAMKC FLLELQVISL ESGDASIHDT VENLIILANN 

       130        140        150        160 
SLSSNGNVTE SGCKECEELE EKNIKEFLQS FVHIVQMFIN TS 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant  IL-15 was lyophilized from a 0.2 μm filtered PBS solution pH 7.5 .

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-15

L15 has been shown to be produced by human fetal astrocytes and microglial cells in response to IL1- beta, IFN-gamma, or TNF- alpha and may thus play a role in T-cell mediated immune responses in the human central nervous system . IL15 stimulates proliferation of the established T-cell line CTLL-2, CD8(+) memory T- cells require IL15 for proliferation.IL15 is also able to induce generation of cytolytic cells and LAK cells activity in vitro. IL15 appears to function as a specific maturation factor for NK-cells and can mimic the bone marrow microenvironment in vitro, which is required for NK- cells to acquire lytic functions. L15 induces proliferation of mast cells in the absence of functional IL2 receptor components and utilizes a receptor of 60-65 kDa that is distinct from known components of T-cell IL15 receptors. The IL15 receptor on mast cells recruits JAK2 and STAT5, instead of JAK1, JAK3 and STAT3 and STAT5 that are activated in T- cells. The alpha subunit of the IL15 receptor (IL15RA) is structurally related to the alpha subunit of the IL2 receptor and confers high affinity binding of IL15 to its receptor. IL15 binds to the beta and gamma chains of the IL2 receptor, which are known to be required for ligand internalization and signal transduction.

Related Publications:
dendritic cells support the in vivo development and maintenance of nk cells via IL-15 trans-presentation
J. Immunol., Sep 2009; 10.4049/jimmunol.0900719.
safety and immunologic effects of IL-15 administration in nonhuman primates
Blood, Sep 2009; 114: 2417 - 2426.
cutting edge: tgf-β1 and IL-15 induce foxp3+ regulatory t cells in the presence of antigen stimulation
J. Immunol., Sep 2009; 183: 3574 - 3577.
antibody-mediated blockade of IL-15 reverses the autoimmune intestinal damage in transgenic mice that overexpress IL-15 in enterocytes
PNAS, Sep 2009; 106: 15849 - 15854.
high antitumor activity of rli, an interleukin-15 (IL-15)–IL-15 receptor fusion protein, in metastatic melanoma and colorectal cancer
Mol. Cancer Ther., Sep 2009; 8: 2736 - 2745.




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