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Human GM-CSF Recombinant (CHO cell)

GM-CSF

accession:  P04141
   Size A     5ug   $ 160
   Size B      20ug  $480
   Size C      1mg  $ 3500
Domain  :  four helix cytokine core
Gene  :  CSF2
Catalog no. :  RCP04141
Source:
Optimized DNA sequence encoding Human Granulocyte Macrophage-Colony Stimulating Factor mature chain was expressed in CHO Cells.

 
Molecular weight:

Native human Granulocyte Macrophage-Colony Stimulating Factor is generated by the proteolytic removal of the signal peptide and propeptide,this molecule has a calculated mass of 15 kDa.

Recombinant GM-CSF is a disulfide-linked monomer  protein, consisting of 126 amino acids and due to glycosylation migrates as an approximately 25 kDa protein under reducing conditions.


 
Purity:
>96%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MWLQSLLLLG TVACSISAPA RSPSPSTQPW EHVNAIQEAR RLLNLSRDTA AEMNETVEVI 

        70         80         90        100        110        120 
SEMFDLQEPT CLQTRLELYK QGLRGSLTKL KGPLTMMASH YKQHCPPTPE TSCATQIITF 

       130        140 
ESFKENLKDF LLVIPFDCWE PVQE 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Human GM-CSF was lyophilized from a 0.2 μm filtered solution in PBS, pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

GM-CSF

GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the aminoterminal end. The sugar moiety is not required for the full spectrum of biological activities. Non- glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non- glycosylated protein. This protein is secreted together with other factors by T-cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM- CSF.

Related Publications:
GM-CSF regulates intimal cell proliferation in nascent atherosclerotic lesions
J. Exp. Med., Sep 2009; 206: 2141 - 2149.
the transcription factors stat5a/b regulate GM-CSF-mediated granulopoiesis
Blood, Sep 2009; 10.1182/blood-2009-04-216390.
GM-CSF and il-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through m-csf receptor shedding by up-regulation of tnf- converting enzyme (tace)
Blood, Sep 2009; 10.1182/blood-2009-04-215020.
biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation
PNAS, Sep 2009; 106: 15825 - 15830.
stress response genes are suppressed in mouse preimplantation embryos by granulocyte-macrophage colony-stimulating factor (GM-CSF)
Hum. Reprod., Sep 2009; 10.1093/humrep/dep307.




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